Journal: International Journal of Molecular Sciences

Article Title: Genetic Background and Kinetics Define Wound Bed Extracellular Vesicles in a Mouse Model of Cutaneous Injury

doi: 10.3390/ijms22073551

Figure Lengend Snippet: Antibodies used for analysis of EV surface markers using vesicle flow cytometry.

Article Snippet: CD18 , M18/2 , APC , 130-104-019 , Miltenyi Biotec.

Techniques: Cytometry

Journal: Fundamental Research

Article Title: A dynamic peripheral immune landscape during human pregnancy

doi: 10.1016/j.fmre.2022.06.011

Figure Lengend Snippet: Aberrant cell-cell communication supported the decreased adaptive immune response after pregnancy. (a) Dot plot showing the representative GO biological process and pathways enriched in downregulated DEGs in DC and MC subsets. (b) Venn plot showing the integrated comparative analysis of downregulated DEGs among DC and MC subsets. (c) Circle plot showing the inferred MHC-II signaling networks. (d) Violin plot showing the expression of genes related to MHC-II signaling among immune populations between HC (blue) and Preg (red) groups. (e) Circle plot showing the inferred CD40 signaling networks. (f) Circle plot showing the inferred ICOS signaling networks in Preg group but absent in HC group. (g) Violin plot showing the expression of genes related to ICOS signaling among immune populations between HC (blue) and Preg (red) groups. (h) Circle plot showing the inferred MIF signaling networks. (i) Violin plot showing the expression of genes related to MIF signaling among immune populations between HC (blue) and Preg (red) groups. (j) The flow cytometry histogram showing the expression of CD74 in CD4 + TCs. Box plots showing the ratio of CD74-expressing CD4 + TCs between HC and Preg groups. (k) Violin plot showing the expression of genes related to ICAM signaling among immune populations between HC (blue) and Preg (red) groups. (l) The flow cytometry histogram showing the expression of CD18 in CD4 + TCs (left). Box plots showing the Mean Fluorescence Intensity (MFI) of CD18 in CD4 + TCs between HC and Preg groups (right). Significance in j, l was calculated using an unpaired, two-tailed Student's t-test; * P < 0.05.

Article Snippet: For the surface staining, PBMCs were simultaneously stained with anti-human fluorochrome-labeled antibodies specific for CD45 (BV785, #304048), CD3 (BV421, #300434), CD14 (PerCP/Cy5.5, #301824), CD4 (APC, #317416 or PE, #300508), CD8 (PerCP/Cy5.5, #301031), CD45RA (BV785, #304140), CD45RO (BV605, #304238), CD1C (PerCP/Cy5.5, #331514), CD19 (BV605, #302244), CD20 (APC, #302310), CD38 (BV785, #303530), HLA-DQ (PE, #318105), BCMA (PE-Cy7, #357508), CD74 (APC, #326811), CD69 (BV650, #310934), CD83 (PE, #305307) (BioLegend, San Diego, USA) and CD18 (APC, #E-AB-F1057E) (Elabscience).

Techniques: Expressing, Flow Cytometry, Fluorescence, Two Tailed Test

Journal: Toxicology and applied pharmacology

Article Title: Carbon monoxide inhalation increases microparticles causing vascular and CNS dysfunction

doi: 10.1016/j.taap.2013.09.019

Figure Lengend Snippet: Neutrophil activation. Data show fold-elevations over control for cell surface presence of myeloperoxidase (MPO), CD18 and CD41 on neutrophils identified by expression of CD66b. Mice were exposed for 1 h to 50, 100, or 1000 ppm CO or to 1000 ppm CO for 40 min and 3000 ppm for 20 min; and then killed immediately or 90 min after exposures. The bar graphs depict proportions as percent of CD66b positive cells expressing greater than 10 arbitrary fluorescence units. The numbers below each segment in the figure show fold-change in CD66b positive cell geometric mean fluorescence (GM) ± SE for each marker. The symbol (*) indicates p < 0.05 versus control.

Article Snippet: R-PE-conjugated anti-mouse CD41 was purchased from e-Biosciences (San Diego, CA), PerCP Cy5.5-conjugated anti-human CD66b from Biolegend (San Diego, CA) and Alexa 647-conjugated anti-mouse CD18 from Serotec (Raleigh, NC).

Techniques: Activation Assay, Control, Expressing, Fluorescence, Marker

Journal: Toxicology and applied pharmacology

Article Title: Carbon monoxide inhalation increases microparticles causing vascular and CNS dysfunction

doi: 10.1016/j.taap.2013.09.019

Figure Lengend Snippet: Neutrophil activation in mice injected with PEG. Data show mean ± SE fold-elevations over those found in cells from control air-breathing mice for cell surface markers as described in . Values in (%) columns indicate fold-change of CD66b positive cells expressing a mean fluorescence value ≥10 arbitrary units for each surface marker. Values in (GM) columns show fold change in CD66b positive cell geometric mean fluorescence. The first row of data labeled PEG shows values from air-breathing mice (no CO exposure) injected with PEG and killed 60 min later. The second row shows neutrophil activation in mice injected with PEG then exposed to 1000 ppm CO for 1 h and killed immediately after the exposure. The third row shows data from mice exposed to 1000 ppm CO for 1 h, then injected with PEG and killed 90 min later.

Article Snippet: R-PE-conjugated anti-mouse CD41 was purchased from e-Biosciences (San Diego, CA), PerCP Cy5.5-conjugated anti-human CD66b from Biolegend (San Diego, CA) and Alexa 647-conjugated anti-mouse CD18 from Serotec (Raleigh, NC).

Techniques: Activation Assay, Injection, Control, Expressing, Fluorescence, Marker, Labeling